Thermo Fisher Scientific Vimentin (Mesenchymal Cell Marker) Monoclonal Antibody (SPM576)
Vimentin 단백질을 인식하는 SPM576 클론의 단클론 항체로, mesenchymal cell marker로 사용됨. WB, IHC, Flow Cytometry 등 다양한 응용 가능. 인간 등 여러 종에 반응하며 Mouse 및 Rat에는 비반응성. 연구용으로만 사용.
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Thermo Fisher Scientific Vimentin (Mesenchymal Cell Marker) Monoclonal Antibody (SPM576)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Cat, Goat, Human, Pig |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM576 |
| Immunogen | Human vimentin recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Product Specific Information
Does not react with Mouse or Rat.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific IFPs. It plays a role in steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation by protein kinase triggers filament breakdown and cytoskeletal rearrangements, facilitating cholesterol transport to mitochondria and enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송 정보
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