Thermo Fisher Scientific NR4A1 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 20 µg/mL

Product Specifications

Product Specific Information

Percent identity with other species by BLAST analysis:

• Human, Orangutan, Gibbon, Monkey, Horse (100%)
• Gorilla, Dog, Bat, Panda (94%)
• Marmoset, Bovine (88%)
• Mouse, Rat (82%)

For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily.
NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung, and prostate.
Its expression is induced in response to various stress stimuli and growth factors.
The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, and also plays a role in signaling in the hypothalamic-pituitary axis.
Translocation of the protein from the nucleus to mitochondria induces apoptosis.
Three transcript variants encoding two distinct isoforms have been identified for NR4A1.
Variant (3), also known as TRC beta, contains multiple differences compared to variant 1.
The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a.
RXR has been shown to be a partner for NR4A1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.