Thermo Fisher Scientific CDK4 Monoclonal Antibody (6D7)

Applications

Western Blot (WB)

• Tested Dilution: 1–5 µg/mL

ELISA

• Tested Dilution: 3 ng/mL

Product Specifications

Product Specific Information

Sequence of this protein is as follows:
KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR DVSLPRGAFP PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKDEG NPE

Target Information

CDK4/cyclin D3 serine/threonine kinase is a dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and plays a key role in controlling cell proliferation during the G1 phase of the cell cycle. It forms a complex with D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2).
CDK4 mediates phosphorylation of the C-terminal region of Rb protein, leading to transcriptional repression of the E2F complex. CDC37 and HSP90 preferentially associate with CDK4 not bound to D-type cyclins. SMAD3 is a major physiological substrate of CDK4 and CDK2.
Although CDK6 and CDK4 can both phosphorylate multiple residues in Rb protein, they exhibit different residue selectivity in vitro: CDK6 phosphorylates Thr821, while CDK4 phosphorylates Thr826.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.