Thermo Fisher Scientific CDK4 Monoclonal Antibody (3E7)

Applications

Western Blot (WB)

• Tested Dilution: 1–5 µg/mL

ELISA

• Tested Dilution: 0.3 ng/mL

Product Specifications

Product Specific Information

Sequence of this protein is as follows:
KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR DVSLPRGAFP PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKDEG NPE

Target Information

CDK4/cyclin D3 serine/threonine kinase is a dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of the cell cycle.
CDK4 forms a complex with D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). It mediates phosphorylation of the C-terminal region of Rb protein, leading to transcriptional repression of the E2F complex.
CDC37 and HSP90 preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of CDK4 and CDK2.
Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they exhibit different residue selectivity in vitro; CDK6 phosphorylates Thr821, while CDK4 phosphorylates Thr826.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.