Thermo Fisher Scientific IFN gamma Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Neutralization (Neu)

Assay-dependent

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant rat IFN-gamma.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

RRID

AB_2536415

Product Specific Information

This antibody neutralizes natural and recombinant rat IFN-y. Cross-reactivity to human IFN-y is less than 0.01% of the homologous combination. Biological Activity: >2.5 x 10^4 neutralizing units/mg, where one neutralizing unit is defined as the amount of antibody sufficient for neutralizing one unit recombinant rat IFN-y (~150 pg) in an antiviral activity bioassay.

For immunohistochemistry applications, it is recommended that the reconstituted antibody be dialyzed against PBS to remove trehalose.

Storage and reconstitution: store the lyophilized antibody at 2-8ºC. Reconstitute using 0.5 mL sterile distilled water. Upon reconstitution, the antibody may be stored at 2-8ºC for up to several weeks. For maximal stability, apportion the reconstituted antibody into working aliquots and store at -20ºC, avoiding repeated freeze/thaw cycles. 0.02% sodium azide may be added to prevent bacterial growth.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.