Thermo Fisher Scientific IFN gamma Monoclonal Antibody (DB-1 (DB1))

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

10 µg/mL

View 16 publications 16 publications

Neutralization (Neu)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 9 publications 9 publications

Product Specifications

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

DB-1 (DB1)

Immunogen

Rat/Mouse IFN-gamma.

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Request custom conjugation

Form

Lyophilized

Storage conditions

4° C

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

RRID

AB_2536414

Product Specific Information

DB-1 exhibits high affinity binding for both rat and mouse natural and recombinant IFN-y and effectively neutralizes the antiviral activity of IFN-y in both mouse and rat. This antibody blocks induction of Ia antigen expression on murine keratinocytes.This antibody is suitable for in vivo and in vitro neutralizing studies. It does not react with rodent IFN-a, rodent IFN-b, or with human interferons.

Biological activity: 6000 neutralizing units/mg of protein. One unit is equivalent to the amount of antibody required to neutralize one laboratory unit of rat IFN-y antiviral activity, in which 6x10^6 units corresponds to 1 mg of pure rat interferon-y. For use in ELISA, this antibody is suitable as a coating antibody when diluted to 10 µg/mL and 100 µl per well.

Storage and reconstitution: store lyophilized antiobdy at or below 4ºC. Reconstitute using 0.5 mL sterile distilled water. Upon reconstitution, apportion into working aliquots and store at or below -20ºC, avoiding repeated freeze/thaw cycles. For long term storage, sodium azide should be added as a preservative.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.