Thermo Fisher Scientific Alkaline Phosphatase Polyclonal Antibody
소, 알칼리성 포스파타제에 특이적인 토끼 폴리클로날 항체로 WB, ELISA, IP에 사용 가능. 동결건조 형태로 제공되며 장기 보관 시 -20°C 이하에서 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:4,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Alkaline Phosphatase (Calf intestine) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 85 mg/mL |
| Storage Buffer | 0.01 M sodium phosphate / whole serum, pH 7.2, with 0.15 M NaCl |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only before immediate use.
Assay Results:
Immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alkaline Phosphatase (Calf intestine). Cross-reactivity with other species or tissue forms may occur but has not been determined. No reaction observed against bacterial enzyme forms.
Target Information
Alkaline phosphatase is a homodimeric enzyme (86 kDa) containing two zinc atoms per monomer, essential for catalytic function. It is active in alkaline pH environments and is found in both prokaryotes and eukaryotes.
- Plays a role in liver metabolism and skeletal development.
- In bacteria, acts as a source of inorganic phosphate.
- In yeast, useful for studying membrane protein sorting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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