Thermo Fisher Scientific Phospho-c-Myc (Ser62) Recombinant Rabbit Monoclonal Antibody (ST49-08)
Phospho-c-Myc (Ser62) 특이적 항체로 Western blot, IHC, ICC/IF에 적합. 재조합 토끼 단일클론으로 높은 특이성과 재현성을 제공. 세포 증식 및 종양 관련 연구에 활용 가능. 안정적인 단백질 A 정제 및 장기 보관 용이.
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Thermo Fisher Scientific Phospho-c-Myc (Ser62) Recombinant Rabbit Monoclonal Antibody (ST49-08)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ST49-08 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser62 of Human c-Myc (aa 46–87) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809463 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone antibody DNA sequences from immunoreactive rabbits and screen individual clones for optimal production.
Key advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the extensive rabbit immune repertoire
Target Information
The c-Myc protein (62 kDa) is a transcription factor encoded on human chromosome 8q24. It is frequently activated in tumor cells and plays a key role in:
- Cellular proliferation, differentiation, apoptosis, and cell cycle progression
- Phosphorylation regulation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK
- Vasculogenesis and angiogenesis in tumor development
Aberrant c-Myc expression is associated with various cancers including lung, breast, colon, neuroblastoma, and Burkitt’s lymphoma.
Antibodies against c-Myc epitopes recognize Myc-tagged proteins at amino or carboxy termini, aiding research into oncogenic mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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