Thermo Fisher Scientific Human IRE1 alpha Synthetic Peptide

Applications

Control (Ctrl)

• Assay-dependent

Blocking Assay (BLOCK)

• Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to 15 amino acids near the amino terminus of human IRE1p.
PEP-0320 can be used as a blocking peptide with polyclonal antibody PA5-20190.

Target Information

ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and endoribonuclease activity. It is important in endoplasmic reticulum-based stress signals and controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells essential for fetal viability.
IRE1 acts as a sensor of unfolded proteins in the ER, initiating transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA, converting it into a potent unfolded-protein response transcriptional activator.
Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through expression of a dominant negative form has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.