Thermo Fisher Scientific Human IRE1 alpha Synthetic Peptide
Human IRE1 alpha 단백질의 카복시 말단 16개 아미노산 서열에 해당하는 합성 펩타이드. 폴리클로날 항체 PA5-20189의 블로킹 펩타이드로 사용 가능. 정제된 액상 형태로 제공되며 PBS 버퍼에 0.1% BSA와 0.02% sodium azide 포함. 연구용으로만 사용.
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Applications
Control (Ctrl)
- Assay-dependent
Blocking Assay (BLOCK)
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Class | Synthetic |
| Type | Peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.2, with 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
Product Specific Information
This peptide corresponds to 16 amino acids near the carboxy terminus of human IRE1p.
PEP-0319 can be used as a blocking peptide with polyclonal antibody PA5-20189.
Target Information
ERN1 serine/threonine kinase, also known as endoplasmic reticulum to nucleus signaling 1, is a human homologue of the yeast Ire1 gene product.
It possesses intrinsic kinase and endoribonuclease activities and plays a key role in endoplasmic reticulum-based stress signaling.
ERN1 regulates IRE1 proteolysis in mammalian cells and is critical for fetal viability.
IRE1 acts as a sensor of unfolded proteins in the ER, initiating transmittance of the unfolded protein signal to the nucleus by splicing XBP1 mRNA, which converts it into a potent transcriptional activator.
Null mutants are viable under normal conditions but essential under stress conditions that cause unfolded protein accumulation in the ER.
Depletion of IRE1p through expression of a dominant negative form does not affect transfected cells under normal conditions but leads to apoptosis under stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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