Thermo Fisher Scientific L1CAM Monoclonal Antibody (OTI1A8), TrueMAB
L1CAM 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, ICC/IF, Flow cytometry 등에 사용 가능. Human 시료 반응성. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용. 신경 발달 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A8 |
| Immunogen | Full length human recombinant protein of human L1CAM produced in HEK293T cells (Target Family: CD171 (L1CAM), UniProt ID: P32004-1, Antigen Range: 1–1257, Antigen Length: 1257) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
L1CAM (CD171) is an axonal glycoprotein belonging to the immunoglobulin supergene family.
Its ectodomain contains multiple immunoglobulin-like and fibronectin type III domains linked to a conserved cytoplasmic domain.
L1CAM plays a crucial role in neuronal migration and differentiation during nervous system development.
Mutations in the L1CAM gene cause X-linked neurological syndromes collectively known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia, and hydrocephalus).
Alternative splicing of a neuron-specific exon is functionally relevant.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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