Thermo Fisher Scientific CD158a/h/g Monoclonal Antibody (HP-MA4), PE, eBioscience

Thermo Fisher Scientific CD158a/h/g Monoclonal Antibody (HP-MA4), PE, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• APC
• eFluor 450
• PE-Cyanine7
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The monoclonal antibody HP-MA4 recognizes several CD158 proteins, also known as KIR2D, specifically KIR2DL1 (CD158a), KIR2DS1 (CD158h), KIR2DS3, and KIR2DS5 (CD158g).
It does not recognize KIR2DL2 (CD158b1), KIR2DS2 (CD158j), KIR2DL4 (CD158d), or KIR2DS4 (CD158i).
Crossreactivity to KIR2DL3 (CD158b2) and KIR2DL5 (CD158f) are unknown.

CD158 proteins are members of the killer cell immunoglobulin-like receptors (KIR) family of transmembrane glycoproteins expressed on NK cells and subsets of T cells.
Each KIR has an isoform with either a short (S) or long (L) cytoplasmic domain, transducing activating or inhibitory signals, respectively.

Applications Reported: HP-MA4 antibody is used for flow cytometric analysis.
Applications Tested: Pre-titrated and tested for flow cytometric analysis of normal human peripheral blood cells.
Recommended use: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells.
KIR genes are polymorphic and highly homologous, located on chromosome 19q13.4 within the leukocyte receptor complex (LRC).
KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by the length of their cytoplasmic domain (L or S).

• Long (L): inhibitory signal via ITIM motif
• Short (S): activating signal via TYRO protein association

Ligands for several KIR proteins are subsets of HLA class I molecules, playing a key role in immune regulation.

For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.

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