Thermo Fisher Scientific SV40 T-Antigen Monoclonal Antibody (PAb416)
SV40 T-Antigen을 인식하는 Mouse monoclonal antibody로, WB, IHC, ICC 등 다양한 응용에 적합합니다. SV40 large 및 small T antigen 검출에 사용되며, 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–5 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1–5 µg/mL | - |
| Immunoprecipitation (IP) | 1–5 µg/mL | - |
| In vitro Assay (IV) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Virus |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PAb416 |
| Immunogen | Full length protein (purified) (SV40) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.05M sodium phosphate with 0.2% gelatin |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_1960836 |
Product Specific Information
This antibody recognizes the 94 kDa SV40 large T antigen and the 20.5 kDa SV40 small T antigen. Recommended positive controls include SV80 or SV-T2 cells.
Target Information
The SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA and complexes with the 53 kDa cellular protein p53, which is required for initiation of viral DNA replication during lytic growth. Additionally, the large T antigen binds DNA polymerase and transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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