Thermo Fisher Scientific SV40 T-Antigen Monoclonal Antibody (PAb416)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

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Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1-5 µg/mL

-

Immunoprecipitation (IP)

1-5 µg/mL

-

In vitro Assay (IV)

-

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Product Specifications

Species Reactivity

Virus

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

PAb416

Immunogen

Full length protein (purified) (SV40).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.05M sodium phosphate with 0.2% gelatin

Contains

0.1% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_1960836

Product Specific Information

This antibody recognizes the 94 kDa SV40 large T antigen and the 20.5 kDa SV40 small T antigen. Recommended positive controls include SV80 or SV-T2 cells.

Target Information

The SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition, the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.