Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Brilliant Ultra Violet 661, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.25 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• APC
• Biotin
• Brilliant UV 615, 737, 805
• Brilliant Violet 421, 650, 786
• Functional Grade
• NovaFluor Red 700, 755
• NovaFluor Yellow 690, 700
• PE, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 780
• Request custom conjugation

Product Specific Information

The MIH5 monoclonal antibody reacts with mouse B7-H1 (PD-L1), a member of the B7 family (~40 kDa) belonging to the Ig superfamily. It is expressed on T, B, NK, and dendritic cells and serves as a ligand for PD-1. The MIH5 clone functions as a blocking antibody.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes, ≤ 0.25 µg/test (100 µL total volume)
• Recommended titration: Optimize empirically for cell numbers (10⁵–10⁸ cells/test)

Fluorochrome Information:
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is suitable for cytometers with a 355 nm UV laser. Ensure instrument compatibility.

Staining Panel Considerations:
When using multiple polymer dye-conjugated antibodies, apply Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation Compatibility:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) with minimal brightness loss.

Excitation / Emission / Laser:
350 nm / 660 nm / Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Programmed death receptor ligand 1 (PD-L1, B7-H1) binds to PD-1, negatively regulating T-cell receptor signaling and reducing proliferation and cytokine production. PD-L1 expression is abundant in murine and human cancers and upregulated by IFN-γ, suggesting a role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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