Thermo Fisher Scientific PAX5 Monoclonal Antibody (7D3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1 µg/mL

-

ELISA (ELISA)

1:10,000

-

ChIP assay (ChIP)

2.5 µg/10^6 cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7D3

Immunogen

Purified recombinant fragment of human PAX5 (amino acids: 235-382) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PAX5,uniProtId:Q02548-1,ncbiNodeId:9606,antigenRange:235-382,antigenLength:391,antigenImageFileName:MA5-17143_PAX5_Q02548-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17143_PAX5_Q02548-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538614

Product Specific Information

MA5-17143 targets PAX5 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples.

The MA5-17143 immunogen is purified recombinant fragment of human PAX5 (amino acids: 235-382) expressed in E. Coli.

MA5-17143 detects PAX5 which has a predicted molecular weight of approximately 45kDa.

Target Information

PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.