Thermo Fisher Scientific PAX5 Recombinant Rabbit Monoclonal Antibody (4I0X4)
PAX5 단백질을 표적으로 하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합. 인간 및 생쥐 반응성. 고순도 친화 크로마토그래피 정제, 안정적 액상 형태로 제공.
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Thermo Fisher Scientific PAX5 Recombinant Rabbit Monoclonal Antibody (4I0X4)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4I0X4 |
| Immunogen | Synthetic peptide corresponding to amino acids 200–300 of human PAX5 (Q02548) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.38 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2912069 |
Product Specific Information
- Positive test controls include: Raji cells
- Immunogen sequence:
DEGIQESPVP NGHSLPGRDF LRKQMRGDLF TQQQLEVLDR VFERQHYSDI FTTTEPIKPE QTTEYSAMAS LAGGLDDMKA NLASPTPADI GSSVPGPQSY P
Target Information
PAX5 is a member of the paired box (PAX) family of transcription factors. PAX proteins play crucial roles in early development, and alterations in their expression are associated with neoplastic transformation.
PAX5 functions as a B-cell lineage-specific activator protein (BSAP), expressed at early but not late stages of B-cell differentiation. It is also detected in developing CNS and testis, suggesting roles in neural development and spermatogenesis.
Mutations in the PAX5 gene can result in leukemia and acute lymphoblastic disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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