Thermo Fisher Scientific eIF4A1 Recombinant Rabbit Monoclonal Antibody (7C3)
Recombinant rabbit monoclonal antibody targeting human eIF4A1 (clone 7C3). Validated for WB and ICC/IF applications. High specificity and purity via affinity chromatography. Supplied in PBS buffer with glycerol and sodium azide for stable storage.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7C3 |
| Immunogen | A synthesized peptide derived from human eIF4A1 (aa 6–20) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.35 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 150 mM NaCl |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2898222 |
Product Specific Information
This antibody has been tested in direct ELISA.
Target Information
eIF4A2 is a 407 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes. Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates genomic RNA synthesis by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most normal tissues, but in variable amounts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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