Thermo Fisher Scientific KLF4 Recombinant Rabbit Monoclonal Antibody (3A1)
인간 KLF4 단백질을 인식하는 재조합 토끼 단클론 항체로, WB, IHC, ICC/IF, IP 등 다양한 응용에 적합. 고순도 친화 크로마토그래피 정제, 액상 형태로 제공되며 안정적인 저장 조건을 지원. 줄기세포 및 세포분화 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 |
| Immunoprecipitation (IP) | 1:200–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3A1 |
| Immunogen | A synthesized peptide derived from human KLF4 (aa 341–355) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 150 mM NaCl |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2898224 |
Product Specific Information
This antibody has been tested in direct ELISA.
Target Information
Kruppel-like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. Also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF), it belongs to the conserved Kruppel-like factor family of DNA-binding zinc finger transcription factors.
KLF4 plays key roles in cellular pathways such as endothelial signaling, adipogenesis, differentiation, embryogenesis, and cell cycle control.
As one of the four Yamanaka factors, it is crucial for reprogramming differentiated cells into pluripotent stem cells. Nuclear localization is associated with oncogenic activity, while cytosolic localization correlates with tumor-suppressive effects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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