Thermo Fisher Scientific CD47 Monoclonal Antibody (OTI6A6)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI6A6

Immunogen

Full length human recombinant protein of human CD47 (NP_001768) produced in HEK293T cell. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD47,uniProtId:Q08722-1,ncbiNodeId:9606,antigenRange:1-323,antigenLength:323,antigenImageFileName:CF813326_CD47_Q08722-1_House_mouse.svg,antigenImageFileNamePDP:CF813326_CD47_Q08722-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 8% trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.