Thermo Fisher Scientific Claudin 2 Polyclonal Antibody (MH44)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25 - 2 µg/mL

View 64 publications 64 publications

Immunohistochemistry (IHC)

Assay-dependent

View 21 publications 21 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:100

View 43 publications 43 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

0.1-1.0 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Dog, Human, Rat

Published species

Dog, Fish, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Clone

MH44

Immunogen

Synthetic peptide derived from the C-terminus of the human Claudin-2 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Claudin 2,uniProtId:P57739-1,ncbiNodeId:9606,antigenRange:230,antigenLength:230,antigenImageFileName:51-6100_Claudin_2_P57739-1_Rabbit.svg,antigenImageFileNamePDP:51-6100_Claudin_2_P57739-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533911

Product Specific Information

This antibody is specific for the ~ 22 kDa Claudin-2 protein and does not appear to cross-react with related endogenous proteins. Reactivity was confirmed by western blotting with rat kidney and by immunostaining of rat small intestine. This antibody is confirmed reactive with human, rat, and dog Claudin-2. The reactivity of this antibody with other species has not been determined.

Target Information

Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. In freeze-fracture electron micrographs, tight junctions are visualized as belt-like bands of anastomosing sealing strands (TJ strands) that completely encircle the lateral surfaces of each cell. TJ strands on adjacent cells are presumed to interact with each other to form a sort of molecular gasket that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other. In addition to this so-called barrier function, the fence function of tight junctions plays an important role in maintaining epithelial cell-polarity by blocking the diffusion of membrane proteins between apical (luminal) and basolateral cell surfaces. Confinement of, for example, the glucose symport to apical surfaces allows glucose to be transported vectorially from the lumen, through the cell, and into the bloodstream. Several peripheral membrane proteins are associated with tight junctions including ZO-1, ZO-2, ZO-3 (members of membrane-associated guanylate-kinase family), cingulin, the 7H6 antigen, Rab-3b, symplekin. While their precise functions are not known, roles for these proteins have been suggested in tight junction assembly and maintenance; signal transduction; and the regulation of tight junction permeability. Furthermore, a growing body of evidence suggests that actin filaments play a major role in regulating tight junction permeability. Until recently, the only transmembrane protein known to be associated with tight junctions was occludin, an ~65 kDa protein with four transmembrane domains. Despite widespread expectation, a critical structural role for occludin in TJ strands was ruled out by the observation of apparently normal tight junctions formed between cells disrupted at both occludin alleles. Fortunately, a closer examination of isolated tight junctions uncovered two related ~22 kDa, four-transmembrane domain proteins, claudin-1 and claudin-2, with no similarity to occludin. In contrast to occludin, which induces only a small number of short strands at cell-cell contact sites when introduced into fibroblasts lacking tight junctions, claudin-1 and -2 induce networks of strands characteristic of true tight junctions. Though inconclusive, these findings suggest that claudin-1 and -2 are major structural components of TJ strands and that occludin plays some other accessory role. Excitement in the tight junction field continues to rise following the recent discovery of claudins -3, -4, -5, -6, -7, and -8 and experiments suggesting that tight junctions in different tissues are comprised of different sets of claudin family proteins. Claudin-1 and claudin-2 connect to the actin cytoskeleton through ZO-1; Claudin-2 functions as a paracellular channel with cation (Na+) selectivity at tight junctions. The expression of claudin-2 is restricted to the liver and kidney, with small amounts also found in the brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.