Thermo Fisher Scientific CD74 Chimeric Recombinant Rabbit Monoclonal Antibody (LN-2)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Specificity: LN-2 antibody recognizes CD74. It reacts with an epitope residing within 60 amino acids of the extracytoplasmic, COOH terminus of the protein.
CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).
The alpha chain gene contains 5 exons:

• Exon 1 encodes the leader peptide
• Exons 2 and 3 encode the two extracellular domains
• Exon 4 encodes the transmembrane domain and the cytoplasmic tail

DRA does not have polymorphisms in the peptide binding region and acts as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5.
Within the DR molecule, the beta chain contains all the polymorphisms specifying the peptide binding specificities.
Hundreds of DRB1 alleles have been described, and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.

HLA-DR is expressed primarily on antigen-presenting cells such as:

• B lymphocytes
• Monocytes
• Macrophages
• Thymic epithelial cells
• Activated T lymphocytes

Three loci—DR, DQ, and DP—encode the major expressed products of the human class II region.
Human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and play a critical role in the initiation of the immune response.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.