Thermo Fisher Scientific IL5RA Polyclonal Antibody

Applications

• Western Blot (WB)

Tested Dilution: 0.1 µg/mL
Publications: -

Product Specifications

Product Specific Information

In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) IL-5 R alpha is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-4 R, rhIL-9 R, rhIL-13 R alpha 1, and rhIL-13 R alpha 2 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cell replacing factor (TRF), that induces B cell and eosinophil growth and differentiation.
IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains:

• Alpha chain: binds IL-5 with low affinity.
• Beta chain: does not bind IL-5 but forms the high-affinity receptor complex with the alpha chain.

The cytoplasmic domain of both chains is essential for signal transduction.
The membrane-proximal proline-rich sequence of the IL-5R alpha subunit is critical for IL-5–induced proliferative response, expression of nuclear proto-oncogenes, and tyrosine phosphorylation of cellular proteins such as JAK1 and JAK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.