Thermo Fisher Scientific CDK2 Monoclonal Antibody (OTI2D9), TrueMAB
CDK2 단백질을 인식하는 마우스 모노클로날 항체로, Western blot, ICC/IF, Flow Cytometry에 적합합니다. 인간, 개, 랫드 반응성을 가지며, 동결건조 형태로 제공됩니다. 연구용으로 세포 주기 조절 관련 연구에 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2D9 |
| Immunogen | Full length human recombinant protein of human CDK2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CDK2/cyclinO is a member of the cyclin-dependent protein kinase family complexed to CyclinO. CyclinO is a specific isoform of the UNG gene localized in the nucleus and exhibits uracil-DNA glycosylase activity.
CDK2/cyclinO protein levels increase during the G1 phase and decrease throughout the cell cycle. Cyclin A–CDK2 levels peak at the G1/S transition, with both proteins associated with DNA during replication initiation. Rb protein acts as a substrate for CDK2–Cyclin E and/or CDK2–Cyclin A in vivo. CDK2 is activated and localized to the nucleus during late G1, S, and G2 phases.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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