Thermo Fisher Scientific MCM2 Polyclonal Antibody
Rabbit polyclonal antibody targeting human MCM2 protein for WB, IHC, IP applications. Recognizes residues 1–50 of MCM2. Provided in liquid form at 1 mg/mL concentration. Recommended storage at 4°C and shelf life of 1 year.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 1 and 50 of human minichromosomal maintenance deficient 2 (MCM2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also known as CDCL1, mitotin, and BM28) is a human nuclear protein crucial for the cell cycle, particularly the onset of DNA replication and cell division. It is similar to early S-phase proteins and has been mapped to chromosome 3q21. CDCL1 is a candidate oncogene affected by chromosomal breaks in acute myeloid leukemia (AML).
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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