Thermo Fisher Scientific MCM3 Polyclonal Antibody
Rabbit polyclonal antibody targeting human MCM3 protein, validated for WB, IHC, and IP. High dilution efficiency and strong specificity for replication-related studies. Provided in liquid form (1 mg/mL), stored at 4°C, suitable for research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 500 and 550 of human Minichromosome Maintenance 3 (MCM3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may play a role in replication fork formation and recruitment of other DNA replication-related proteins.
This protein is a subunit of the MCM2–7 complex and interacts directly with MCM5/CDC46. It is also acetylated by MCM3AP, a chromatin-associated acetyltransferase. Acetylation inhibits initiation of DNA replication and cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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