Thermo Fisher Scientific Dynactin 1 Monoclonal Antibody (6B11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:1,750

View 1 publication 1 publication

Immunoprecipitation (IP)

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

6B11

Immunogen

Synthetic peptide corresponding to the N-terminal of human Dynactin 1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Dynactin 1,uniProtId:Q14203-1,ncbiNodeId:9606,antigenRange:1,antigenLength:1278,antigenImageFileName:MA5-18308_Dynactin_1_Q14203-1_House_mouse.svg,antigenImageFileNamePDP:MA5-18308_Dynactin_1_Q14203-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2539681

Product Specific Information

This antibody detects endogenous levels of DCTN1 at a molecular weight of 150 kDa and does not cross-react with related proteins.

Purity is >95% by SDS-PAGE.

Target Information

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.