Thermo Fisher Scientific LIN7A Monoclonal Antibody (P879)
LIN7A 단백질을 인식하는 마우스 모노클로날 항체로, Western blot, ICC/IF, IP에 사용 가능. 인간 시료에 반응하며 단기 4°C, 장기 -20°C 보관. 단백질 복합체 연구 및 세포막 단백질 분포 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | P879 |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 12–227 of LIN7A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| RRID | AB_2539679 |
Product Specific Information
- Recommended positive controls: MCF-7, MDA-MB-231
- Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (97%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
LIN7A plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. It forms membrane-associated multiprotein complexes that regulate delivery and recycling of proteins to correct membrane domains.
The tripartite complex composed of LIN7 (LIN7A, LIN7B, or LIN7C), CASK, and APBA1 may couple synaptic vesicle exocytosis to cell adhesion in the brain.
LIN7A ensures proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses, recruited by beta-catenin and cadherin.
It is required to localize Kir2 channels, GABA transporter (SLC6A12), and EGFR/ERBB1, ERBB2, ERBB3, and ERBB4 to the basolateral membrane of epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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