Thermo Fisher Scientific KLF4 Recombinant Rabbit Monoclonal Antibody (ARC0721)
KLF4 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체입니다. WB, IHC, ICC, ELISA, IP 등 다양한 실험에 적용 가능하며, 높은 특이성과 재현성을 제공합니다. 인간, 마우스, 랫트 반응성. 연구용으로만 사용.
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Thermo Fisher Scientific KLF4 Recombinant Rabbit Monoclonal Antibody (ARC0721)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0721 |
| Immunogen | Synthetic peptide corresponding to amino acids 300–400 of human KLF4 (O43474) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20 °C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897766 |
Product Specific Information
- Positive samples: Mouse lung, HeLa, A-549, A-431
- Immunogen sequence:
PAAHDFPLGR QLPSRTTPTL GLEEVLSSRD CHPALPLPPG FHPHPGPNYP SFLPDQMQPQ VPPLHYQGQS RGFVARAGEP CVCWPHFGTH GMMLTPPSSP L
Target Information
Kruppel-like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. Also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF), KLF4 belongs to a conserved family of DNA-binding zinc finger transcription factors.
It plays a pivotal role in cellular reprogramming as one of the four Yamanaka factors, contributing to pluripotency induction. KLF4 is involved in endothelial signaling, adipogenesis, differentiation, embryogenesis, and cell cycle control.
Its localization determines biological function—nuclear localization is associated with oncogenic activity, while cytosolic localization correlates with tumor suppression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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