Thermo Fisher Scientific KLF4 Recombinant Rabbit Monoclonal Antibody (ARC0721)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC0721

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human KLF4 (O43474) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KLF4,uniProtId:O43474-3,ncbiNodeId:9606,antigenRange:300-400,antigenLength:513,antigenImageFileName:MA5-37846_KLF4_O43474-3_Rabbit.svg,antigenImageFileNamePDP:MA5-37846_KLF4_O43474-3_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.8 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897766

Product Specific Information

Positive Samples: Mouse lung, HeLa, A-549, A-431

Immunogen sequence: PAAHDFPLGR QLPSRTTPTL GLEEVLSSRD CHPALPLPPG FHPHPGPNYP SFLPDQMQPQ VPPLHYQGQS RGFVARAGEP CVCWPHFGTH GMMLTPPSSP L

Target Information

Kruppel like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. It is also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF) and belongs to a family of conserved Kruppel-like factor family of DNA binding zinc finger transcription factors. KLF4 exerts its functional relevance across varied cellular pathways. As one of the four Yamanaka factors, it plays a key role in reprogramming differentiated cells into pluripotent stem cells. Most studies have focused on its specific role in endothelial cell signaling, adipogenesis, differentiation, embryogenesis and cell cycle control. Localization plays an important role in the biological significance of this protein wherein nuclear localization is associated with its function as an oncogene and cytosolic localization with tumor suppression specific effect.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.