Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine5, eBioscience

Applications

Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV Series (395–805)
• Brilliant Violet Series (421–786)
• eFluor Series (450–660)
• FITC
• Functional Grade
• PE, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright Series (436–780)
• Custom conjugation available upon request

Product Specific Information

The eBio299Arm monoclonal antibody serves as an isotype control immunoglobulin.

• Applications Reported: Suitable for flow cytometric analysis.
• Applications Tested: Verified for use in flow cytometry; should be used at the same concentration as the experimental antibody.
• Excitation: 488–561 nm
• Emission: 667 nm
• Laser Compatibility: Blue, Green, Yellow-Green lasers
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Isotype control antibodies help distinguish specific antibody signals from non-specific background noise.
They prevent misinterpretation caused by:

• Fc receptor binding
• Non-specific interactions with cellular components
• Autofluorescence

Usage:

• Primarily used in flow cytometry
• Also applicable in ChIP, immunohistochemistry, and gel shift assays
• Should match the species and isotype of the primary antibody
• For directly labeled antibodies, use isotype controls conjugated with the same fluorophore

Note:
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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