Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), Super Bright 436, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 605
• Brilliant Violet 711
• eFluor 450
• eFluor 570
• eFluor 660
• FITC
• NovaFluor Red 710
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM and does not cross-react with other mouse immunoglobulin classes (IgD, IgG, IgA). IgM is expressed intracellularly in early B lymphopoiesis and on the surface of mature B cells in bone marrow and peripheral tissues. Fluorochrome-conjugated II/41 can be used as a detection secondary for mouse IgM.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤1.0 µg/test (in 100 µL final volume). Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Fluorochrome characteristics:
Super Bright 436 is excited with a 405 nm violet laser and emits at 436 nm. Recommended detection filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Mouse IgM is an immunoglobulin M antibody produced by B cells in response to antigen exposure. It is the first antibody produced during an immune response and the largest immunoglobulin in serum. Composed of five heavy and five light chains, IgM mediates complement activation and antigen agglutination, playing a key role in innate immunity. It is widely used in research and diagnostics, including ELISA, Western blot, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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