Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), Super Bright 436, eBioscience
CD326 (EpCAM) 단클론 항체(G8.8)는 마우스 EpCAM을 인식하는 Super Bright 436 형광 표지 항체로, 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 405 nm 레이저로 여기되며 436 nm에서 방출됩니다. 암세포 표지 및 면역분석 연구용으로 적합합니다.
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Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), Super Bright 436, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 5 publications |
| Flow Cytometry (Flow) | 1.0 µg/test | View 26 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G8.8 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2744801 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40, 17-1A antigen, TACSTD1, and CD326. The immunogen used was the TE-71 thymic epithelial cell line. CD326 is expressed on most epithelial cells and considered a pan-carcinoma antigen. It mediates calcium-independent, homophilic cell-cell adhesion and may function as a growth factor receptor.
Applications Reported: This G8.8 antibody has been reported for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of the TE-71 cell line. Use ≤1.0 µg per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody should be titrated for optimal performance.
Super Bright 436 is excited with a violet laser line (405 nm) and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent adhesion molecule influencing cell cycle and proliferation via activation of c-myc, cyclin A, and E. Ep-CAM overexpression is associated with increased epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors. It is found on the basolateral surface of most simple epithelia and distinguishes adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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