Thermo Fisher Scientific GTPase HRAS Polyclonal Antibody, FITC
FITC로 표지된 HRAS 단백질에 특이적인 토끼 폴리클로날 항체. Western blot, ELISA, IP에 사용 가능. 인간, 생쥐, 닭 반응성. 합성 펩타이드(aa 100-150) 면역원 사용. 형광 분석용으로 적합하며 -20°C 어두운 곳에 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 100–150 of human GTPase HRAS protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Monomeric G protein Ras acts as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to multiple downstream signaling pathways that regulate cytoplasmic and nuclear events. Mammalian cells contain three Ras proto-oncogenes encoding closely related proteins: H-, K-, and N-Ras. Oncogenic Ras mutations occur in approximately 30% of human cancers. Constitutive Ras activation leads to increased proliferation and reduced apoptosis.
- K-Ras mutations: Common in pancreatic, colorectal, and non-small-cell lung carcinomas
- H-Ras mutations: Common in bladder, kidney, and thyroid carcinomas
- N-Ras mutations: Found in melanoma, hematologic malignancies, and hepatocellular carcinoma
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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