Invitrogen™

PiPer™ Pyrophosphate or Phosphate Assay Kit

PiPer Pyrophosphate Assay Kit provides reagents for fluorogenic or colorimetric detection as low as 0.4 mμM (fluorescence) or 2 μM (absorbance) inorganic pyrophosphate (PPi) while Phosphate Assay kits can detect down to 0.2 μM Pi in purified enzyme systems. Each kit has enough for 1000 labelings.

PiPer Pyrophosphate and Phosphate Assay Kit provides an ultrasensitive assay to detect the fluorescent product resorufin. These kits can be used to detect inorganic pyrophosphate (PPi) or phosphate (Pi) in a variety of samples or can monitor the kinetics of their release by a variety of enzymes, including DNA/RNA polymerases, adenylate cyclase, S-acetyl coenzyme A synthetase, ATPases, GTPases, 5′-nucleotidase, protein phosphatases, acid/alkaline phosphatases and phosphorylase kinase. As resorufin has a strong absorption, this assay can be performed either fluorometrically or colorimetrically.

사양

색상Red

검출 방법Colorimetric, Fluorescence

용도(애플리케이션)Phosphate Assay

용도 (장비)Spectrophotometer, Microplate Reader

제품 유형Phosphate Assay

염료 유형Amplex™ Red

형식96-well plate

제품라인PiPer™

수량1000 Assays

배송 조건Room Temperature

여기/방출563/587 nm

Unit SizeEach