Thermo Fisher Scientific DHFR Monoclonal Antibody (OTI3A11), TrueMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI3A11

Immunogen

Full-length protein expressed in 293T cell transfected with human DHFR expression vector if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DHFR,uniProtId:P00374-1,ncbiNodeId:9606,antigenRange:1-187,antigenLength:187,antigenImageFileName:CF500517_DHFR_P00374-1_House_mouse.svg,antigenImageFileNamePDP:CF500517_DHFR_P00374-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, and is required for the synthesis of purines, thymidylic acid and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.