Thermo Fisher Scientific Phospho-PDPK1 (Tyr9) Polyclonal Antibody
Human 시료에 반응하는 phospho-PDPK1 (Tyr9) rabbit polyclonal antibody. IHC(P)에서 1:50–1:100 희석으로 사용 가능. Affinity chromatography로 정제된 액상 항체로, PBS/glycerol buffer에 보관. PDPK1 연구 및 대사 관련 신호전달 연구에 적합.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 9 (A-L-Y(p)-D-A) derived from Human PDK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554288 |
Product Specific Information
- A suggested positive control for IHC is human brain tissue.
Target Information
PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases and activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop.
It also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate.
Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), leading to the dephosphorylation and activation of glycogen synthase and stimulation of glycogen synthesis.
Because of PDPK1’s role in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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