Thermo Fisher Scientific Human PLK4, GST Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Baculovirus

Amino acid sequence

1-836

Tag

GST-tag

Molecular weight

120.9 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 10mM glutathione, 25% glycerol, 0.1mM PMSF, 0.25mM DTT, 150mM NaCl, 0.1mM EDTA

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Recombinant human PLK4 (1-836) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen™ Eu Kinase Binding Assay

Protein Form: Recombinant, Fragment

The gene accession number for PLK4 is NP_055079.3.

Target Information

PLK4 or polo-like kinase 4 is a member of the polo family of serine/threonine protein kinases which localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. The overexpression of PLK4 triggered the simultaneous formation of multiple procentrioles around each preexisting centriole which resulting in centriole amplification and thus, Plk4-induced centriole biogenesis in human cells. The reduced Plk4 gene dosage increases the probability of mitotic errors and cancer development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.