Thermo Fisher Scientific Human PLK4, GST Tag Recombinant Protein
Recombinant human PLK4 protein with GST tag expressed in baculovirus system. Validated for LanthaScreen™ kinase binding assays. Supplied in liquid form for research use only. Stable at -80°C with optimized storage buffer for enzyme integrity.
- 카탈로그번호
- PV639x (3개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific Human PLK4, GST Tag Recombinant Protein
Applications
- Kinase Assay (KA)
Tested Dilution
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | Baculovirus |
| Amino Acid Sequence | 1–836 |
| Tag | GST-tag |
| Molecular Weight | 120.9 kDa |
| Class | Recombinant |
| Type | Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Purified |
| Storage Buffer | 50 mM Tris HCl (pH 7.5), 10 mM glutathione, 25% glycerol, 0.1 mM PMSF, 0.25 mM DTT, 150 mM NaCl, 0.1 mM EDTA |
| Contains | No preservative |
| Storage Conditions | -80°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
Product Specific Information
For maximum recovery, please spin prior to use.
Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use materials from original packaging until exhausted.
For larger sizes, aliquoting may reduce freeze/thaw cycles; avoid aliquots below 20 µL.
Do not store kinase diluted.
Properly stored at -80°C, product stability is guaranteed for 6 months from purchase.
Recombinant human PLK4 (1–836) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Features:
- Validated in LanthaScreen™ Kinase Binding Assay
- Protocols available for simple use and optimized performance
- Multiple pack sizes for convenient use
Related Product:
LanthaScreen™ Eu Kinase Binding Assay
Protein Form: Recombinant, Fragment
Gene Accession Number: NP_055079.3
Target Information
PLK4 (Polo-like kinase 4) is a serine/threonine protein kinase that localizes to centrioles and regulates centriole duplication during the cell cycle. Overexpression induces multiple procentrioles around existing centrioles, leading to centriole amplification and Plk4-induced centriole biogenesis. Reduced PLK4 expression increases mitotic error risk and cancer development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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