Thermo Fisher Scientific Human PRKACB (PRKAC beta), GST Tag Recombinant Protein

Thermo Fisher Scientific Human PRKACB (PRKAC beta), GST Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution: Assay-dependent
Publications: References

Product Specifications

Product Specific Information

• For maximum recovery, spin prior to use.
• Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use in original packaging until exhausted.
• For larger sizes, aliquots may reduce freeze/thaw cycles (do not prepare aliquots below 20 µL).
• Never store kinase diluted.
• Properly stored at -80°C, guaranteed for 6 months from date of purchase.
• Recombinant full-length human PRKACB expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Validated Applications:

• LanthaScreen™ Kinase Binding Assay
• Protocols available for simple and optimized use
• Multiple pack sizes for convenience

Related Product: LanthaScreen™ Eu Kinase Binding Assay

Protein Form: Recombinant
Gene Accession Number: NP_002722.1

Target Information

The catalytic subunit C-beta of PKA (PRKACB) belongs to the Ser/Thr protein kinase family. The PKA catalytic subunit comprises three gene products: C-alpha, C-beta, and C-gamma, located on human chromosome region 1p36.1.
PRKACB is derived from a distinct gene from C-alpha and exhibits tissue-specific expression. At the amino acid level, C-alpha and C-beta show 93% homology.
Inactive holoenzyme of AMPK forms a tetramer of two regulatory and two catalytic subunits. cAMP induces dissociation into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four regulatory and three catalytic subunits of AMPK have been identified in humans.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.