Thermo Fisher Scientific Dinitrophenyl-KLH Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 16 publications 16 publications

Immunohistochemistry (IHC)

Assay-dependent

View 9 publications 9 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 15 publications 15 publications

Flow Cytometry (Flow)

Assay-dependent

View 2 publications 2 publications

ELISA (ELISA)

-

View 3 publications 3 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Neutralization (Neu)

-

View 4 publications 4 publications

Dot blot (DB)

-

View 1 publication 1 publication

Cytotoxicity Assay (CT)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 7 publications 7 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Dinitrophenyl-KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_221552

Target Information

DNP-KLH antibodies are used for detecting DNP-labeled molecules. Fluorescent and biotinylated anti-DNP-KLH conjugates can serve as second-step reagents for signal amplification in protein and nucleic acid detection systems, including in situ hybridization studies employing DNP-modified DNA probes. Because anti-DNP antibody binds well to DAMP (D-1552), an acidotropic reagent that can penetrate cell membranes, anti-DNP-KLH antibodies can also be used with DAMP for immunochemical localization of intracellular compartments with low pH. DNP-KLH antibodies may recognize compounds with a trinitrophenyl group, allowing the isolation of cell-surface molecules of human melanoma cells that have been labeled with trinitrobenzene sulfonic acid (TNBS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.