Thermo Fisher Scientific Carboxypeptidase Y Monoclonal Antibody (10A5B5)
Thermo Fisher Scientific의 Carboxypeptidase Y Monoclonal Antibody (10A5B5)는 효모 단백질 CPY를 인식하는 마우스 IgG2a 항체로, Western blot 및 면역형광 분석에 적합합니다. Vps-phenotype 선별과 효모 단백질 위치 확인에 사용되며, 고순도 Ig fraction으로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.25 µg/mL | View 41 publications |
| Immunocytochemistry (ICC/IF) | 20 µg/mL | View 2 publications |
| Flow Cytometry (Flow) | - | View 1 publication |
| ELISA | - | View 1 publication |
| Immunoprecipitation (IP) | - | View 9 publications |
| Dot blot (DB) | - | View 2 publications |
| Miscellaneous PubMed (Misc) | - | View 5 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Yeast |
| Published Species | Yeast |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10A5B5 |
| Immunogen | Purified yeast CPY (carboxypeptidase) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ig fraction |
| Storage Buffer | HEPES buffered saline |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_2536203 |
Product Specific Information
Antibody binding specificity was determined by Particle Concentration Fluorescence Immunoassay (PCFIA) using yeast carboxypeptidase, by Western blot using yeast protein extracts, and by indirect immunofluorescence of fixed yeast cells.
This antibody can detect yeast carboxypeptidase on Western blots or immunolocalize it in fixed yeast cells.
It is ideal for screening carboxypeptidase secretion (Vps-phenotype) in yeast using colony immunoblot overlay assays.
The antibody has been widely used in screens for new vps mutations and in complementation analysis with the vps mutant collection.
Target Information
Carboxypeptidase Y is involved in the degradation of small peptides. It digests peptides containing an aliphatic or hydrophobic residue in the P1 position, as well as methionine, leucine, or phenylalanine in the P1 position of an ester substrate.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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