Thermo Fisher Scientific Carboxypeptidase Y Monoclonal Antibody (10A5B5)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25 µg/mL

View 41 publications 41 publications

Immunocytochemistry (ICC/IF)

20 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 9 publications 9 publications

Dot blot (DB)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 5 publications 5 publications

Product Specifications

Species Reactivity

Yeast

Published species

Yeast

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

10A5B5

Immunogen

Purified yeast CPY (carboxypeptidase) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Carboxypeptidase Y,uniProtId:P00729-1,ncbiNodeId:-23,antigenRange:1-532,antigenLength:532,antigenImageFileName:A-6428_Carboxypeptidase_Y_P00729-1_House_mouse.svg,antigenImageFileNamePDP:A-6428_Carboxypeptidase_Y_P00729-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ig fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2536203

Product Specific Information

Antibody binding specificity was determined by Particle Concentration Fluorescence Immunoassay (PCFIA) using the yeast caboxypeptidase, by Western blot immunoassay using protein extracts from yeast, and by indirect immunofluorescence of fixed yeast cells.The antibody can be used to detect the yeast carboxypeptidase on Western blots or to immunolocalize the carboxypeptidase in fixed yeast cells. The antibody is ideal for screening for caroboxypeptidase secretion (Vps-phenotype) in yeast using the colony immunoblot overlay assay. The antibody has been extensively used in screens for new vps mutations, as well as in complementation analysis with the vps mutant collection.

Target Information

Carboxypeptidase Y is Involved in the degradation of small peptides. It digests peptides containing an aliphatic or hydrophobic residue in P1` position, as well as methionine, leucine or phenylalanine in the P1 position of an ester substrate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.