Thermo Fisher Scientific WT1 Monoclonal Antibody (2H4)

Applications

Western Blot (WB)

• Tested Dilution: 1–5 µg/mL

ELISA

• Tested Dilution: 10 µg/mL

Product Specifications

Product Specific Information

Sequence of this protein is as follows:
QDVRRVPGVA PTLVRSASET SEKRPFMCAY PGCNKRYFKL SHLQMHSRKH TGEKPYQCDF KDCERRFSRS DQLKRHQRRH TGVKPFQCKT C

Target Information

The Wilms Tumor 1 (WT1) gene encodes one of the WPP domain-interacting proteins required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. RanGAP is critical for establishing the RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells.

In Arabidopsis thaliana, RanGAP association with the root tip nuclear envelope requires nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). WT1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar but distinct from the WIP family, required for RanGAP nuclear envelope association in root tip cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.