Thermo Fisher Scientific RTN1 Monoclonal Antibody (RNL-4)
Reticulon-1C(NSP-C) 단백질을 검출하는 Thermo Fisher의 Mouse Monoclonal Antibody. Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. Human, Pig, Rat 반응성. 1 mg/mL 농도, PBS buffer 및 sodium azide 포함. 연구용 전용 제품.
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Thermo Fisher Scientific RTN1 Monoclonal Antibody (RNL-4)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1:50–1:100 |
| Immunoprecipitation (IP) | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Pig, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RNL-4 |
| Immunogen | Synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of reticulon-1C |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2285730 |
Product Specific Information
MA1-06815 detects reticulon-1C (NSP-C) in human, porcine, and rat samples.
It has been successfully used in flow cytometry, Western blotting, immunocytochemistry, and immunohistochemistry procedures.
By Western blot, it detects a ~23 kDa band representing reticulon-1C.
The immunogen is a synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of reticulon-1C.
Target Information
Reticulon-1 (NSP) is specifically expressed in neural and neuroendocrine tissues.
The NSP gene is mapped to human chromosome 14q21–q22 and encodes three overlapping proteins:
- Reticulon-1A (NSP-A): ~135 kDa, multiple isoforms depending on serine phosphorylation, marker for neuroendocrine differentiation in lung cancer.
- Reticulon-1B (NSP-B): 45 kDa phosphoprotein, restricted to lung cancer cell line NCI-H82, not found in human tissues.
- Reticulon-1C (NSP-C): 23 kDa, non-phosphorylated, found with Reticulon-1A in SCLC cell lines, absent in non-SCLC cultures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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