Thermo Fisher Scientific Perlecan Monoclonal Antibody (A7L6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:25-1:200

-

Immunocytochemistry (ICC/IF)

1:25-1:200

- -

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Fish, Human, Mouse, Rat

Published species

Bovine, Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

A7L6

Immunogen

High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Perlecan,uniProtId:P98160-1,ncbiNodeId:9606,antigenRange:1-4391,antigenLength:4391,antigenImageFileName:MA1-06821_Perlecan_P98160-1_Rat.svg,antigenImageFileNamePDP:MA1-06821_Perlecan_P98160-1_Rat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_559394

Product Specific Information

MA1-06821 detects large heparan sulphate proteoglycan in human, mouse, bovine, cod, wolffish, and rat samples.

MA1-06821 has sucessfully been used in Western blotting, immunocytochemistry, immunoprecipitation, and immunohistochemistry procedures. By Western blot it detects a >400 kDa band representing large HSPG.

The MA1-06821 immunogen is a high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.

Target Information

Perlecan is a major heparan-sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth factors, perlecan has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.