Thermo Fisher Scientific LY9 Monoclonal Antibody (OTI2H4), TrueMAB
LY9 단백질을 인식하는 Mouse IgG1 단클론 항체로, Human 시료에 반응. Western blot 및 IHC(Paraffin) 실험에 적합하며, 순도 높은 친화 크로마토그래피 정제 제품. CD229(Ly9) 연구용으로 사용되며, 동결건조 형태로 제공.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2H4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 48–454 of human LY9 produced in SF9 cell (CD229 (Ly9), UniProt ID: Q9HBG7-1, antigen range: 48–454) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD229 (Ly9) is a cell surface receptor belonging to the CD150 family, which includes CD48 and CD224. These receptors regulate cytokine production and cytotoxicity in lymphocytes and NK cells.
High levels of CD229 are found on T and B cells, increasing during maturation, and it is absent on granulocytes, dendritic cells, platelets, and erythrocytes.
CD229 is also reported on mouse monocytes and NK cells. It interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen-presenting B cells during immune synapse formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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