Thermo Fisher Scientific Phospho-PI3 kinase p85 alpha/gamma (Tyr467, Tyr199) Polyclonal Antibody
PI3K p85 alpha/gamma 인산화형(Tyr467, Tyr199)을 인식하는 Rabbit Polyclonal 항체로 다양한 응용 가능. Western blot, IHC, ICC, Flow cytometry, ELISA에 적합. 고순도 Protein A 정제, 안정적 액상 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1 µg/test |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human PI3K around the phosphorylation site of p85 alpha Tyr467 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Phosphoinositide 3-kinase (PI3K, phospho PI3K) binds to activated (phosphorylated) protein-Tyr kinases through its SH2 domain and acts as an adapter mediating the association of the p110 catalytic unit to the plasma membrane. Phospho PI3K is necessary for insulin-stimulated glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, coordinating cell growth, cell cycle entry, cell migration, and cell survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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