Thermo Fisher Scientific Tyrosinase Recombinant Mouse Monoclonal Antibody (rOCA1, 812)
Recombinant mouse monoclonal antibody against Tyrosinase for IHC (paraffin). High specificity and sensitivity with protein A/G purification. Suitable for research use only. Stable at 4°C, supplied in PBS with BSA and sodium azide.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): Tested dilution 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rOCA1, 812 |
| Immunogen | Recombinant full-length human Tyrosinase protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
The enzyme encoded by this gene catalyzes the first two steps, and at least one subsequent step, in the conversion of tyrosine to melanin. It possesses both tyrosine hydroxylase and dopa oxidase activities and requires copper for function. Mutations in this gene cause oculocutaneous albinism, while nonpathologic polymorphisms contribute to skin pigmentation variation. The human genome also contains a pseudogene similar to the 3′ half of this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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