Thermo Fisher Scientific Phospho-PKC theta (Ser695) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 641 and 702 of Human PKC theta-phospho-S695 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKC theta,uniProtId:Q04759-1,ncbiNodeId:9606,antigenRange:641-702,antigenLength:706,antigenImageFileName:PA5-29029_PKC_theta_Q04759-1_Rabbit.svg,antigenImageFileNamePDP:PA5-29029_PKC_theta_Q04759-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.66 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2546505

Product Specific Information

Recommended positive controls: HeLa, HepG2, Molt-4, Raji, MCF7 (500nM PMA treatment for 1hr).

Predicted reactivity: Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involve. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu. The different PKC isoforms vary in structure, subcellular localization, tissue distribution and response to extracellular signals. PKC beta II has been shown to be expressed, in part, in cardiac muscle, intestinal epithelium, kidney and pancreas. PKC beta II has been shown to be involved in cell cycle progression and cancer proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.