Thermo Fisher Scientific MCMBP Polyclonal Antibody
MCMBP 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC(P), ICC/IF 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태로, 장기 보관 시 -20°C 저장 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC(P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human MCMBP. Recombinant protein control fragment (Product #RP-97206). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2643815 |
Product Specific Information
Immunogen sequence:
SPRNTTLERQ TFYCVPVPGE STWVKEAYVN ANQARVSPST SYTPSRHKRS
YEDDDDMDLQ PNKQKDQHAG ARQAGSVGGL QWCGEPKRLE TEASTGQ- Highest antigen sequence identity to the following orthologs:
- Mouse: 88%
- Rat: 90%
Target Information
MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases.
MEK1 activates ERK1 and ERK2 MAP kinases.
Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop.
MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK.
Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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