Thermo Fisher Scientific E2F6 Recombinant Rabbit Monoclonal Antibody (ARC0628)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

ChIP assay (ChIP)

5 µg antibody for 10 µg-15 µg of Chromatin

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CUT&RUN (C&R)

105 cells /1 µg

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC0628

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 110-281 of human E2F6 (NP_937987.2) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:E2F6,uniProtId:O75461-1,ncbiNodeId:9606,antigenRange:110-281,antigenLength:281,antigenImageFileName:MA5-37840_E2F6_O75461-1_Rabbit.svg,antigenImageFileNamePDP:MA5-37840_E2F6_O75461-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897760

Product Specific Information

Positive Samples: 293T, HepG2, RD

Immunogen sequence: LDGIDLVEKK SKNHIRWIGS DLSNFGAVPQ QKKLQEELSD LSAMEDALDE LIKDCAQQLF ELTDDKENER LAYVTYQDIH SIQAFHEQIV IAVKAPAETR LDVPAPREDS ITVHIRSTNG PIDVYLCEVE QGQTSNKRSE GVGTSSSEST HPEGPEEEEN PQQSEELLEV SN

Target Information

This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.