Thermo Fisher Scientific Human Leptin Receptor Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

CHO cells

Amino acid sequence

FNLSYPITPW RFKLSCMPPN STYDYFLLPA GLSKNTSNSN GHYETAVEPK FNSSGTHFSN LSKTTFHCCF RSEQDRNCSL CADNIEGKTF VSTVNSLVFQ QIDANWNIQC WLKGDLKLFI CYVESLFKNL FRNYNYKVHL LYVLPEVLED SPLVPQKGSF QMVHCNCSVH ECCECLVPVP TAKLNDTLLM CLKITSGGVI FQSPLMSVQP INMVKPDPPL GLHMEITDDG NLKISWSSPP LVPFPLQYQV KYSENSTTVI READKIVSAT SLLVDSILPG SSYEVQVRGK RLDGPGIWSD WSTPRVFTTQ DVIYFPPKIL TSVGSNVSFH CIYKKENKIV PSKEIVWWMN LAEKIPQSQY DVVSDHVSKV TFFNLNETKP RGKFTYDAVY CCNEHECHHR YAELYVIDVN INISCETDGY LTKMTCRWST STIQSLAEST LQLRYHRSSL YCSDIPSIHP ISEPKDCYLQ SDGFYECIFQ PIFLLSGYTM WIRINHSLGS LDSPPTCVLP DSVVKPLPPS SVKAEITINI GLLKISWEKP VFPENNLQFQ IRYGLSGKEV QWKMYEVYDA KSKSVSLPVP DLCAVYAVQV RCKRLDGLGY WSNWSNPAYT VVMDIKVPMR GPEFWRIING DTMKKEKNVT LLWKPLMKND SLCSVQRYVI NHHTSCNGTW SEDVGNHTKF TFLWTEQAHT VTVLAINSIG ASVANFNLTF SWPMSKVNIV QSLSAYPLNS SCVIVSWILS PSDYKLMYFI IEWKNLNEDG EIKWLRISSS VKKYYIHDHF IPIEKYQFSL YPIFMEGVGK PKIINSFTQD DIEKHQSD

Molecular weight

93.5 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

PeproTech`s Recombinant Human Leptin Receptor is a single, glycosylated polypeptide chain of 818 amino acid residues, corresponding to the human OB-Re isoform, and has a calculated molecular weight of approximately 93.5 kDa. As a result of glycosylation, Recombinant Human Leptin Receptor migrates with an apparent molecular mass of approximately 130-150 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

The leptin (OB-R) receptor is a member of the cytokine receptor superfamily which plays an important role in mammalian body weight homeostasis and energy balance. Ligand-induced activation of the OB-R by the adipose tissue-secreted hormone, leptin, appears to activate downstream signaling events through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. Leptin can homo-dimerize OB-R extracellular domains while downstream signaling events are initiated upon homo-dimerization of OB-R intracellular domains. Several isoforms of the OB-R which differ in length and signaling capabilities have been reported. RNA transcripts encoding the OB-R long form have been found in the choroid plexus and in the hypothalamus which has been proposed as a control center for satiety and energy expenditure. Mutations in either the mouse OB-R (db) or leptin (ob) genes have been shown to result in early-onset obesity. Metabolic abnormalities attributable to these genotypes include hypercorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered CNS activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.