Thermo Fisher Scientific MHC Class I Recombinant Rabbit Monoclonal Antibody (ARC1291)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunocytochemistry (ICC/IF)

1:100-1:400

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC1291

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MHC class I (HLA-A/B) (P04439) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MHC Class I,uniProtId:P04439-1,ncbiNodeId:9606,antigenRange:200-300,antigenLength:365,antigenImageFileName:MA5-35712_MHC_Class_I_P04439-1_Rabbit.svg,antigenImageFileNamePDP:MA5-35712_MHC_Class_I_P04439-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849612

Product Specific Information

Immunogen sequence: KETLQRTDPP KTHMTHHPIS DHEATLRCWA LGFYPAEITL TWQRDGEDQT QDTELVETRP AGDGTFQKWA AVVVPSGEEQ RYTCHVQHEG LPKPLTLRWE L

Target Information

Like their mammalian counterparts, avian MHC Class I molecules, also known as B-F antigens, consist of a highly polymorphic alpha-chain noncovalently bound to the invariant beta2-microglobulin subunit. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. The monoclonal antibody F21-2 also reacts with turkey MHC Class I.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.